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Phytophthora borealis Hansen et al. 拉丁名‘’

(ATCC® MYA-4881™) 統(tǒng)一編號

Classification Peronosporaceae, Phytophthora, borealis

Strain Designations 別名 AKWA58.1-0708 [CBS 132023]

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Type Strain 模式菌種 yes

Preceptrol no

Comments 注釋 Plant pathogen

Morphology After 5 days on V8 juice medium at 20°C colonies are angular and petaloid with hyphae appressed. After 3 weeks sporangia are persistent. Sporangia ovoid or obpyriform.  Oogonia absent.

Medium 培養(yǎng)基 ATCC® Medium 343: V8 juice agar

ATCC® Medium 1970: V8 rye agar

ATCC® Medium 321: Lima bean agar

ATCC® Medium 2232: YM medium with biotin 30 mcg/L and thiamine 600 mcg/L

Growth Conditions 生長條件 

Temperature 培養(yǎng)基: 20°C to 22°C

Atmosphere 需氧情況: Typical aerobic

Sequenced Data GenBank accession number: JQ745261

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATCAACCCCTTAAA

TTT

GGGGGCTTGCTCGGCGGCGTGCGTGCTGGCCTGTAATGGGTCGGCGTGYTGCTGCTGGGCGGGCTCTATCATGG

G

CGAGCGTTTGGGCTTCGGCTCGAGCTAGTAGCTATCAATTTTAAACCCCTTCTTAAATACTGAACATACTGTGGGGAC

GAAAGTCTCTGCTTTTAACTAGATAGCAACTTTCAGCAGTGGATGTCTAGGCTCGCACATCGATGAAGAACGCTGCG

AACTGCGATACGTAATGCGAATTGCAGGATTCAGTGAGTCATCGAAATTTTGAACGCATATTGCACTTCCGGGTTA

GTC、CTGGGAGTATGCCTGTATCAGTGTCCGTACATCAACCTTGGTTTTCTTCCTTCCGTGTAGTCGGTGGAGGATATGCCAGACGTGAAGTGTCTTGCTGGCGGTCTTTCGAGTCTGCCGGTGAGTCCTTTGAAATGTACTGAACTGTACTCTCTCTTTG

CTCGAAAAGCGTGGCGTTGCTGGTTGTGGAGGCTGCCCGTGTGGCCAGTCGGCGACCGGTTTGTTASCTGTGGCGT

TTAATGGAGGAGTGTTYGATTCGCGGTATGGTTGGCTTCGGCTGAACAATCTGCTTATTGGGTGCTTTTCCTGTCA

TTGGCGGTACGAACTGGTGAACCGTAGCTGTGTGGTGCTTGGCTTTTGAACCGGCTTTGCTTTGCGAAGTAGGG

TGGCGG

CTTCGGCTGTCGAGGGGTCGATCCATTTTGGGAAACTTTGTGTGTGCGGCTTCGTGCTGCGCGCATCTCAATTGGA

CCTGATATCAGGCAAGATTACCCGCTGAACTTAAG

GenBank accession number: JQ745268

Large subunit ribosomal RNA gene, partial sequence.

CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCTCAA

GCTTAAAATCTCCGTGCAAGTTTTGCGCGGCGAATTGTAGTCTATAGAGGCGTGGTCAGCGTGGGCGCTTGGG

GCAA

GTTCCTTGGAAGAGGACAGCATGGAGGGTGATACTCCCGTTCATCCCTGAGTGGCTCGTGCGTACGACCCGTGTT

CTTT

GAGTCGCGTTGTTTGGGAATGCAGCGCAAAGTAGGTGGTAAATTCCATCTAAAGCTAAATATTGGTGCGAGACCG

AT

AGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAAGAGTACCTGAAACTGCTGAA

AG

GGAACCGAATCGTTTCCAGTGTCTATAATCCGTGGCATATTTCATTGGCGAGTGTGTGCGCGCGTGCGCTGTGGCAG

CGGCTTTTTTGGCTGCGCTCGGTGCGTGTGTTGTGTGTGCTTGCTGGTGCCCTGTGCTGCGGTGGGACGTCAAGGTC

AGTTCGTATGCTGCGGGAAATGGCTGCCGAGGAGGTAGGGCTTACGCTCTGCGTTTGTCTATTATATCTTGGTGGAC

GAGTCGTCGCGGTTGGGACTGAGGTGCCTACAACGTGCTTTTGAGTGGGTGTGTGTCTCCGTGTGCGCCGTGTGCG

GATAGCTTGCTATGCGTGTGTGGTTGTGTGTGGATTGATGCGTGCCTTAACTTGTCGCCGTTCGGGACGTTGACGAA

ATGGAGCGATCCGAC

Morphology After 5 days on V8 juice medium at 20°C colonies are angular and petaloid with hyphae appressed. After 3 weeks sporangia are persistent. Sporangia ovoid or obpyriform.  Oogonia absent.

Name of Depositor 寄存者 E Hansen

Special Collection ATCC

Chain of Custody 來源國家 ATCC <-- E Hansen <-- P Reeser

Isolation 分離基物 Forest streams, Bear Creek, Alaska, USA

Cross References Nucleotide (GenBank) : JQ745261 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : JQ745268 D1/D2 region of 28S rRNA gene.

References 參考文獻 Hansen EM, et al. Phytophthora borealis and Phytophthora riparia, new species in Phytophthora ITS clade 6. Mycologia 104: 1133-1142. PubMed: 22778166