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Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki 拉丁名

 (ATCC? 58376?) 統(tǒng)一編號(hào)

Deposited As Pichia stipitis Pignal

Strain Designations 別名 NRRL Y-7124 [CBS 5773, CCRC 21775, DBVPG 6264, LY 1321]

Application 用途 Bioethanol production

Biosafety Level 生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏條件 

Frozen 冷凍物: -80°C or colder

Freeze-Dried 凍干物: 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌種 yes

Preceptrol no

Genome Sequenced Strain Yes

Comments 注釋 Taxonomy

Activity of plasma membrane ATPase

Immobilization

Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).

Medium 培養(yǎng)基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar

ATCC? Medium 200: YM agar or YM broth

ATCC? Medium 1245: YEPD

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 20°C to 25°C

Atmosphere 需氧情況: Typical aerobic   

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAAC

CTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTCAGAAATGAGTTGGGCCAGAGGTTTACCAA

ACTTCAATTTTATTGAATTGTTATTTTATTAATTTGTCAATTTGTTGATTAAATTCAAAAATCTTCAAAACTTTCAACAA

CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAAT

CATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAA

CCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCGAACTAGGCGTTTGCTTGAAAAGTATTGGCACGAGTGGTACTAA

ATAGTACTGACAGAATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGACTTCTGGCGGTGAATTTTTGGTATATT

GGCTTTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAAT

AA

D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCT

CAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTCAGCTCTTGT

CTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCTGATTCTATGTAAA

GTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT

ATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAA

AGTACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGG

GCCTCTACAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACCACTTCG

GTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCAT

AATGATCTTAAACCGC

Name of Depositor 寄存者 NRRL

Chain of Custody 來源國家 ATCC <-- NRRL

Isolation 分離基物 Insect larvae, France

Cross References Nucleotide (GenBank) : JQ023145 RPB 2 gene

Nucleotide (GenBank) : JQ023133 RPB 1 gene

Nucleotide (GenBank) : HQ652060 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : HQ651939 18S rRNA gene

Nucleotide (GenBank) : KC479699 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KC507454 EF-1 alpha gene

Nucleotide (GenBank) : KC479707 XYL1 gene

References 參考文獻(xiàn) Toivola A, et al. Alcoholic fermentation of D-xylose by yeasts. Appl. Environ. Microbiol. 47: 1221-1223, 1984.

Calleja GB, et al. Rapid process for the conversion of xylose to ethanol. Canadian Patent 1,266,245

Tantirungkij M, et al. Construction of xylose-assimilating Saccharomyces cerevisiae. J. Ferment. Bioeng. 75: 83-88, 1993.

Roberto IC, et al. Utilization of sugar cane Bagasse hemicellulosic hydrolysate by Pichia stipitis for the production of ethanol. Process Biochem. 26: 15-21, 1991.

Billon-Grand G. A new ascosporogenous yeast genus: Yamadazyma gen. nov.. Mycotaxon 35: 201-204, 1989.

Meyrial V, et al. Ethanol tolerance and activity of plasma membrane ATPase in Pichia stipitis grown on D-xylose or on D-glucose. Enzyme Microb. Technol. 17: 535-540, 1995.

Pignal MC. Une nouvelle espece de levure isolee de larves d'insectes: Pichia stipits. Bull. Mens. Soc. Linn. Lyon 36: 163-168, 1967.

Slininger PJ, et al. Growth, death, and oxygen uptake kinetics of Pichia stipitis on xylose. Biotechnol. Bioeng. 37: 973-980, 1991.

Targonski, Z. Biotransformation of lignin-related aromatic-compounds by Pichia stipitis. Zentralbl. Mikrobiol. 147: 244-249, 1992.

. . Proc. Eur. Cong. Biotechnol. 6: 1035-1038, 1994.

Webb SR, Lee H. Characterization of xylose reductase from the yeast Pichia stiipitis: evidence for functional thiol and histidyl groups. J. Gen. Microbiol. 138: 1857-1863, 1992.

Nigam JN. Ethanol production from hardwood spent sulfite liquor using an adapted strain of Pichia stipitis. J. Ind. Microbiol. Biotechnol. 26: 145-150, 2001. PubMed: 11420654

Slininger PJ, et al. Comparative evaluation of ethanol production by xylose-fermenting yeasts presented high xylose concentrations. Biotechnol. Lett. 7: 431-436, 1985.

Bjorling T, Lindman B. Evaluation of xylose-fermenting yeasts for ethanol production from spent sulfite liquor. Enzyme Microb. Technol. 11: 240-246, 1989.

Delgenes JP, et al. Acid hydrolysis of wheat straw and process considerations for ethanol fermentation by Pichia stipitis Y7124. Process Biochem. 25: 132-135, 1990.

Takuma S, et al. Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 28-29: 327-340, 1991. PubMed: 1929370

Kurtzman CP, Suzuki M. Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience 51: 2-14, 2010.

Shields S, Boopathy R. Ethanol production from lignocellulosic biomass of energy cane. Int. Biodeterior. Biodegrad. 65: 142-146, 2011.

Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624