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平臺編號：bio-81915
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國際編號：ATCC 37808
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拉丁屬名： pMMB206 in Escherichia coli
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pMMB206
pMMB206 in Escherichia coli 拉丁名
(ATCC? 37808?) 編號
Designations 菌株別名 pMMB206 in Escherichia coli
Depositors V Morales, National Research Council, Plant Biotechnology Institute, Saskatoon, Sask., Canada
Biosafety Level 生物安全等級 1
Host Escherichia coli TB1
Vector Information Size (kb): 9.31
Vector: pMMB206 (plasmid)
Promoters: Promoter tac
Construction: pMMB190, pUC8
Marker(s): cmlR
Construct size (kb): 9.31
Features: insert detection: lacZ' marker(s): cmlR promoter: tac, lacUV5 replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB
Applications 用途 expression vector shuttle vector
Comments 注釋 Restriction digests of the clone give the following sizes (kb): EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9; EcoRI/MluI--8.4, 0.9.
Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation.
Shows a low level of expression without induction. Sequencing demonstrated that there are 3 stop codons in frame with the truncated bla gene, terminating translation but permitting continued transcription and expression of the cat gene.
Media 培養(yǎng)基 ATCC? Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 37°C
References 參考文獻 Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347
Shipped 提供形式 frozen
ATCC37808
