Ogataea polymorpha (Morais et Maia) Yamada, Maeda et Mikata
MYA-336 ™
Download GenomeLearn about the ATCC Genome Portal An ampoule containing viable cells suspended in cryoprotectant.
Product categoryFungi
Product typeYeast
Strain designationNCYC 495 leu1.1 ura3Type strainNoGenome sequenced strainYesIsolation sourceDerived from NCYC 495, ATCC 14754ApplicationsFood production research
Product formatFrozenStorage conditions-80°C or colder
General
Specific applicationsNon-conventional yeasts for bioconversion and precision fermentation
PreceptrolNo
Characteristics
PloidyHaploidGenotypeleu1.1 ura3MorphologyAfter 3 days at 25°C colonies are butyrous and tannish-white in color, cells are spheroidal to elongate, (1.5-4.6)x(1.9-5.3) um, and single, in pairs, or in small clusters.
MediumATCC Medium 2797: YPD w/ 30mg/L Leucine and 30mg/L uracil
Temperature24-26°C
AtmosphereAerobic
Handling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
