Clostridium difficile (Hall and O'Toole) Prevot 拉丁名
(ATCC? 43593?) 統(tǒng)一編號
Strain Designations 菌種別名 1351
Isolation 分離基物 Feces, human, Belgium
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Serogroup B
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen(冷凍物): -80℃ or colder
Freeze-Dried(凍干物): 2℃ to 8℃
Live Culture: See Propagation Sectio
Preceptrol? no
Type Strain 模式菌種 no
Antigenic Properties Serogroup B
Comments 注釋 Toxinotype tcdA-, tcdB- Ribotype 060 Binary toxin gene cdtB not amplified by PCR 、
Medium 培養(yǎng)基 ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37℃
Atmosphere 需氧情況 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor 寄存人 M Delmee
Isolation 分離基物 Feces, human, Belgium
References 參考文獻 Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688
Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303