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Hs 832(C).T [Hs832.Tc]

(ATCC? CRL-7566?)

Organism Homo sapiens, human

Tissue ovary/benign cyst

Product Format frozen Morphology fibroblast

Culture Properties adherent

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease endometriosis Gender female Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Derivation The line was established from a benign ovarian cyst taken from a patient with endometriosis. The cells were isolated from both the inner and outer surfaces of the cyst. The line senesces after passage 28.

Clinical Data Caucasian female Comments Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description. Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium,

Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.

Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37℃. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Cryopreservation

Freeze medium: 50% FBS, 40% Growth Medium, 10% DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃

Population Doubling Time 72 hrs Passage History We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

The line senesces after passage 28. Year of Origin 1974