Product categoryFungi
Product typeNucleic acid
Derived fromAspergillus niger 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI 45551, J. Friedrich A98, KCC F-0086, NRRL 334, QM 324, QM 458, Steinberg, TC 215-4247, WB 334] (ATCC 6275)
OrganismAspergillus niger van Tieghem
Type strainNoGenome sequenced strainYesApplicationsNext-generation sequencing
Mass2 μgProduct formatFreeze-dried
Characteristics
CommentsThis preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR) process and other molecular biology applications.
Handling information
Handling procedureCentrifuge tube prior to opening to prevent loss of pelleted material
DNA is dried in 1X TE buffer. Rehydrate contents of vial with a desired amount of molecular grade water or any preferred buffer. Resuspending the dried DNA in ≥250 μL may give better results.
Place vial at 37°C for 1 hour, or at 2°C to 8°C overnight.
For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking.
To enhance PCR efficiency, add 1 μL of freshly prepared dry milk powder solution (50 mg/mL) to a PCR mix (25 to 50 μL). PCR with “hot start” is also recommended for better results.
Handling notesGenomic DNA is appropriate for PCR and other molecular biology applications.
Quality control specifications
Electrophoresis - RNA contentNo RNA was detected by electrophoresisPurity (A260/A280)1.7 to 2.1IntegrityIntegrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.Functional testsFunctional activity was confirmed by PCR amplification of approximately 1500 base pairs fragment of rRNA gene cluster including ITS1-5.8S-ITS2 region.IdentityIdentity confirmed by sequencing of ITS1, 5.8S gene and ITS2 regions of ribosomal RNA (~ 500 base pairs).Verification methodWhole-genome Sequencing
ATCC6275D-2
