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Clostridium acetobutylicum McCoy et al. emend. Keis et al. 拉丁名

(ATCC? 824?)  統(tǒng)一編號

Deposited As Granulobacter pectinovorum (Stormer) Beijerinck

Strain Designations 菌株別名 [CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787]

Application  用途 Assay of 4-aminobenzoic acid p-aminobenzoic acid Degrades xylan Produces acetone Produces acidolysin Produces autobacteriocin Produces butyl alcohol butanol Produces ethyl alcohol ethanol Produces restriction endonuclease Cac824I Transformation host Isolation Plant-derived foodstuff, cornmeal

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏條件

Frozen（冷凍物）: -80℃ or colder

Freeze-Dried（凍干物）: 2℃ to 8℃

Live Culture（活物）: See Propagation Section

Preceptrol? no

Genome Sequenced Strain Clostridium acetobutylicum ATCC 824, complete genome

Type Strain 模式菌株 yes

Comments  注釋 Genome sequenced strain Verification of identity Agglutination

Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)

ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度 : 37℃

Atmosphere 需氧情況 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2

Name of Depositor 寄存人  ER Weyer

Chain of Custody 來源國家 ATCC <-- ER Weyer

Isolation 分離基物 Plant-derived foodstuff, cornmeal

References  參考文獻 Lee SF, et al. Xylanolytic activity of Clostridium acetobutylicum. Appl. Environ. Microbiol. 50: 1068-1076, 1985.

Soucaille P, Goma G. Acetonobutylic fermentation by Clostridium acetobutylicum ATCC 824: Autobacteriocin production, properties, and effects. Curr. Microbiol. 13: 163-169, 1986.

Croux C, et al. Purification and characterization of acidolysin, an acidic metalloprotease produced by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 56: 3634-3642, 1990. PubMed: 2082818

Validation list no. 51. Int. J. Syst. Bacteriol. 44: 852, 1994. J. Infect. Dis. 56: 333-346, 1935.

Mermelstein LD, et al. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Bio-Technology 10: 190-195, 1992. PubMed: 1368230

Ash C, et al. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253-260, 1993. PubMed: 8085788

Wilkinson SR, et al. Phenotypic and genotypic differences between certain strains of Clostridium acetobutylicum. FEMS Microbiol. Lett. 125: 199-204, 1995.

Boynton ZL, et al. Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. J. Bacteriol. 178: 3015-3024, 1996. PubMed: 8655474

Boynton ZL, et al. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutlicum ATCC 824. Appl. Environ. Microbiol. 62: 2758-2766, 1996. PubMed: 8702268

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Girdwood RH. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33, 1963. PubMed: 13948188

Lopez-Contreras AM, et al. Utilisation of saccharides in extruded domestic organic waste by Clostridium acetobutylicum ATCC 824 for production of acetone, butanol and ethanol. Appl. Microbiol. Biotechnol. 54: 162-167, 2000. PubMed: 10968627

Keis S, et al. Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov.. Int. J. Syst. Evol. Microbiol. 51: 2095-2103, 2001. PubMed: 11760952

Bryant DL, Blaschek HP. Buffering as a means for increasing growth and butanol production by Clostridium acetobutylicum. J. Ind. Microbiol. 3: 49-55, 1988.

Cross References Nucleotide (GenBank) : X78070 C. acetobutylicum (ATCC 824, T) 16S rRNA

Nucleotide (GenBank) : X70334 C. acetobutylicum gene for starch degrading enzyme

Nucleotide (GenBank) : M68865 Clostridium acetobutylicum autolytic lysozyme (lyc) gene, complete cds

Nucleotide (GenBank) : AF205034 Clostridium acetobutylicum sucrose utilization operon, complete sequence.

Nucleotide (GenBank) : M96945 Clostridium acetobutylicum butanol dehydrogenase isozyme A gene, complete cds

Nucleotide (GenBank) : M96946 Clostridium acetobutylicum butanol dehydrogenase isozyme B gene, complete cds

Nucleotide (GenBank) : AE001438 Clostridium acetobutylicum ATCC 824 megaplasmid pSOL1, complete sequence

Nucleotide (GenBank) : S77333 thl=thiolase {5' region} [Clostridium acetobutylicum, ATCC 824, Genomic, 81 nt]

Nucleotide (GenBank) : U16166 Clostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequence

Nucleotide (GenBank) : AF047822 Clostridium acetobutylicum catabolic acetolactate synthase (alsS) gene, complete cds

Nucleotide (GenBank) : U38234 Clostridium acetobutylicum phosphotransacetylase (pta) and acetate kinase (ack) genes, complete cds

Nucleotide (GenBank) : AF321779 Clostridium acetobutylicum plasmid pSOL1 aldehyde/alcohol dehydrogenase (adhE2) gene, complete cds

Nucleotide (GenBank) : L14744 Clostridium acetobutylicum phosphotransbutyrylase, complete cds; butyrate kinase, complete cds and ORF1

Nucleotide (GenBank) : L14817 Clostridium acetobutylicum transcriptional repressor (solR) and aldehyde-alcohol dehydrogenase (aad) genes, complete cds

Nucleotide (GenBank) : U52366 Clostridium acetobutylicum phosphofructokinase (pfk) gene, complete cds, and pyruvate kinase homolog (pyk) gene, partial cds

Nucleotide (GenBank) : M93363 Clostridium acetobutylicum acetoacetate decarboxylase, acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase subunit, complete cds

Nucleotide (GenBank) : U08465 Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase gene, complete cds, and homolog of B. subtilis orfX gene, partial cds

Nucleotide (GenBank) : U15277 Clostridium acetobutylicum putative regulatory protein and putative ortho phosphoribosyl transferase (pyrE) genes, partial cds and hydrogenase I (hydA) gene, complete cds

Nucleotide (GenBank) : U52368 Clostridium acetobutylicum rubredoxin oxidoreductase homolog and deformylase (def) genes, complete cds, and methionyl-tRNA formyltransferase homolog (fmt) gene, partial cds

Nucleotide (GenBank) : U07420 Clostridium acetobutylicum ATCC 824 putative sigma factor E (sigE) gene, putative sigma factor E processing enzyme (ORFA), gene and putative sigma factor G (sigG) gene, complete cds

Nucleotide (GenBank) : U52367 Clostridium acetobutylicum ATCC 824 F-type ATP synthase subunit a (atpA) gene, F-type ATP synthase subunit c (atpC) gene, and F-type ATP synthase subunit b (atpB) gene, complete cds

Nucleotide (GenBank) : U17110 Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA dehydrogenase

Nucleotide (GenBank) : AE001437 Clostridium acetobutylicum ATCC 824, complete genome