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Murine pneumonia virus

VR-1819 ™

Product category

Viruses

Product type

Animal virus

Classification

Paramyxoviridae, Pneumovirus

Strain designation

15

Isolation source

Lungs of mice

Product format

Frozen

Storage conditions

-70°C or colder

Characteristics

Comments

ATCC VR-1819 was treated for Mycoplasma sp. at the ATCC by three passages with media supplemented with an antibiotic (Mycoplasma Removal Agent – Bio-Rad catalog BUF035).  The original item number was ATCC VR-25.

Handling information

Host

BHK-21 [C-13] (ATCC CCL-10)

Effects

CPE; cell degeneration; cell rounding

Complete medium

EMEM (ATCC 30-2003) + 10% FBS (ATCC 30-2020)

Temperature

37°C

Atmosphere

95% Air, 5% CO2

Incubation

5-12 days at 37°C in a humidified 5% CO2atmosphere, until CPE is progressed through 80% of the monolayer.

Recommendation for infection

For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 2-3 hours at 37°C in a humidified 5% CO2atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Handling notes

ATCC VR-1819 was treated for Mycoplasma sp. at the ATCC by three passages with media supplemented with an antibiotic (Mycoplasma Removal Agent – Bio-Rad catalog BUF035).  The original item number was ATCC VR-25.

Key abbreviations

°C, Degrees Celsius

BHK-21, Hamster kidney cell line

CO2, Carbon dioxide

CPE, Cytopathic effect

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

PBS, Phosphate-buffered saline

Quality control specifications

Volume

1.0 mL

Verification method

Whole-genome Sequencing

History

Deposited as

Murine pneumonia virus

Depositors

J Parker

Type of isolate

Animal

Year of origin

1939

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.