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平臺編號：bio-50967
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國際編號：ATCC VR-14
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拉丁屬名： Human adenovirus D
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
中文名稱暫無
Human adenovirus D (ATCC VR-14 )
Classification Adenoviridae, Mastadenovirus
Agent Human adenovirus D
Strain A.A.
Common Name Human adenovirus 13
Applications
Respiratory research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Recognized Reference Strain: Pereira HG, et al.A short description of adenovirus group. Virology 20: 613, 1963.
Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter. Contains soluble group-specific complement fixing antigen.
Effect on Host
CPE, rounding and sloughing
Recommended Host
KB cells (ATCC
CCL-17
)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 60-90% confluent. Remove medium and inoculate with a small volume (e.g. 1mL per 25 cm squared) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 1-2 hours at 37°C in a 5% CO2 atmosphere. End adsorbtion by adding VGM.
Incubation: Incubate infected culture for 1-6 days at 37°C in a 5% CO2 humidified atmosphere until CPE is well advanced through 90% of culture.
Effect on Host
CPE, rounding and sloughing
Name of Depositor RJ Huebner, NIH
Chain of Custody
Deposited as Adenovirus type 13
Source Anal swab from healthy child, Washington, DC, 1955
References
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
Rowe WP, et al. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97: 465-470, 1958. PubMed: 13518306
Classification Adenoviridae, Mastadenovirus
Agent Human adenovirus D
Strain A.A.
Common Name Human adenovirus 13
Applications
Respiratory research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Recognized Reference Strain: Pereira HG, et al.A short description of adenovirus group. Virology 20: 613, 1963.
Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter. Contains soluble group-specific complement fixing antigen.
Effect on Host
CPE, rounding and sloughing
Recommended Host
KB cells (ATCC
CCL-17)Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 60-90% confluent. Remove medium and inoculate with a small volume (e.g. 1mL per 25 cm squared) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 1-2 hours at 37°C in a 5% CO2 atmosphere. End adsorbtion by adding VGM.
Incubation: Incubate infected culture for 1-6 days at 37°C in a 5% CO2 humidified atmosphere until CPE is well advanced through 90% of culture.
Effect on Host
CPE, rounding and sloughing
Name of Depositor RJ Huebner, NIH
Chain of Custody
Deposited as Adenovirus type 13
Source Anal swab from healthy child, Washington, DC, 1955
References
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
Rowe WP, et al. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97: 465-470, 1958. PubMed: 13518306
