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平臺編號：bio-50150
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國際編號：ATCC VR-3163
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
中文名稱暫無
ATCC? Number:VR-3163?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts67
Depositors: S. Dales, R. Condit
Biosafety Level:2
Shipped: frozen
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Host Organism : Production host: BSC-40 (ATCCCRL-2761)Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate number 8125, was isolated from the IHD-W strain of Vaccinia by S. Dales, selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. This mutant was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. The non-permissive incubation temperature is 39.5oC. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Related Products: ATCC VR-1441 Vaccinia
References : 33180: Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 61241: Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 16172602: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at
