ATCC? Number:VR-3149?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts45
Depositors: S. Dales, R. Condit
Biosafety Level:2
Shipped: frozen
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Host Organism : Production host: BSC-40 (ATCCCRL-2761)Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Store at : -70.0℃ or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 6754, was isolated from the IHD-W strain of Vaccinia by S. Dales. Not described in the original publication, it is presumed to have been selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5℃. This mutant was not assigned to a Dales EM category. Mutations were located by complementation analysis (Lackner, et al, 2003) within vaccinia map locations A30 and G(6-8). Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Related Products: Virus: ATCC VR-1441
References : 33180: Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 61241: Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 16172602: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at