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平臺(tái)編號(hào)：bio-50140
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國(guó)際編號(hào)：ATCC VR-3153
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
中文名稱暫無(wú)
ATCC? Number:VR-3153?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts66
Depositors: S. Dales, R. Condit
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production host: BSC-40 (ATCCCRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Incubation : For best results, plate cells 24-48 hours in advance in DMEM with 10% FBS at 37.0°C. Infect at 90-100% confluence using a multiplicity of infection (MOI) of about 1. Infect and incubate in DMEM supplemented with 2% FBS at 31.0°C for 1-3 days. Harvest cells and culture fluids when 100% of culture shows advanced cytopathic effects. Vaccinia virus is largely cell associated. Concentrated preparations can be made by collection and sonication of cell pellets.
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 7954, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5°C. The mutant was not assigned to a Dales EM category. Its mutation was assigned by complementation analysis (Lackner, et al, 2003) to map location A24, the 132-kDa viral RNA polymerase subunit. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Related Products: Virus: ATCC VR-1441
