ATCC? Number:VR-3108?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant deposited as Vaccinia virus, Orthopoxvirus
Strain: IHD-W Dts41
Depositors: S Dales, RC Condit
Biosafety Level:2 [CDC BMBL recommends vaccination Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses [16172602] ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects include enlargement and rounding of cells, followed by death and eventual sloughing.
Store at : -70 C or lower
Comments : Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location E(2-8). [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W