ATCC? Number:VR-3158?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts91
Depositors: S dales, R Condit
Biosafety Level:2 [BSL 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
Store at : -70 C or lower
Comments : Non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, Q. This mutant was eliminated during Complementational analysis.
Related Products: VR-1441 (Vaccinia virus, strain: IHD-W)