ATCC? Number:VR-2430?
Classification: Arteriviridae
Agent: Porcine respiratory and reproductive syndrome virus
Strain: ISU-55-SAH (plaque purified)
Original Source: pneumonic pig lungs, United States
Depositors: Iowa State Univ. Res. Fndn.
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Temperature: 37.0°C Duration: 2-3 days at 37C in PSP-36-SAH cells
Effect : Yes, in vitro effects: Cytopathic effects (degeneration, rounding, and clumping of cells)
Store at : -70°C (or colder)
Comments : This material is bacterially contaminated.This virus produces respiratory and reproductive disease in pigs. Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome. Inoculate 2-3 day-old confluent monolayers with undiluted or 1:10 dilution of virus stock. After 1 hour add culture medium containing 2% FBS.
References : 38246: Paul PS, et al. Highly virulent porcine reproductive and respiratory syndrome viruses which produce lesions in pigs and vaccines that protect pigs against SAID syndrome. US Patent 5,695,766 dated Dec 9 1997