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Mus musculus murine
    Mus musculus murine
  • 平臺(tái)編號(hào):bio-49956
  • 國(guó)際編號(hào):ATCC VR-1518AF-MAB?
  • 拉丁屬名: Mus musculus murine
  • 規(guī)格:freeze-dried
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

ATCC? Number:VR-1518AF-MAB?
Antiserum Against: Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod Agent: Mus musculus murine Strain: monoclonal antibody against FOS peptide (359-378) [LA 054]
Biosafety Level:1
Shipped: freeze-dried
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Store at : Vials may be stored at 2-8 C.
Comments : Immunogen: Synthetic peptide RKGSSSNEPSSDSLSSPTLL(C) (residues 359-378 of the FOS sequence) coupled to keyhole limpet hemocyanin Source and Sex of Animals: Male 129GIX+ mice were used for immunization. Fusion Partners: 1.3 x 10(8) nucleated spleen cells with 2 x 10(7) SP2/0 non-secreting myeloma cells Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr. Immunization Schedule: Approximately 70 mg of coupled peptide in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and complete Freund's adjuvant administered intraperitoneally on day 0; followed by an equal amount of conjugate in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and 10 mg/ml alum administered intraperitoneally on day 14; followed by 70 mg of coupled peptide in 200 ml of phosphate buffered saline pH 7.4 administered intravenously on day 51. The spleen was removed on day 56. Preparation: Monoclonal antibody is in ascites fluid collected from 2-06-87 to 7-06-87. The ascites fluid was ammonium sulfate precipitated, reconstituted, filter sterilized, aliquoted 1 ml per vial, and lyophilized. The sterility is not guaranteed. Resuspend in 1 ml distilled water. Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay Vials may be stored at 2-8擄C. IgG1


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