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OSU (virulent, gut origin)
    OSU (virulent, gut origin)
  • 平臺編號:bio-49867
  • 國際編號:ATCC VR-892
  • 拉丁屬名: Porcine rotavirus
  • 規(guī)格:frozen
  • 用途:ATCC原裝進口
  • 服務費用:
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  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產品信息以出庫為準)

ATCC? Number:VR-892?
Classification: Reoviridae, Rotavirus
Agent: Porcine rotavirus
Strain: OSU (virulent, gut origin)
Original Source: clinical specimen - animal (intestinal contents of one- to four-week-old pigs with diarrhea)Ohio, United States
Depositors: EH Bohl
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Sus scrofa
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 36.0°C Duration: 4-7 days with some rocking; For best results cells should be 24 to 72 hours old and lightly 100% confluent. Pretreatment of virus with trypsin is needed to activate virus before inoculation.
[Note: Do not use serum in the culture medium.]
Effect : Yes, in vitro effects: Cytopathic effects (some rounding, cell degeneration and sloughing) with addition of pancreatin or trypsin at a level slightly under a toxic dose for the monolayer Yes, in vivo effects: diarrhea in small pigs
Store at : -70°C (or colder)
Comments : Serotype 5 (G5) by antigenic analysis of VP7 and serotype P7 by antigenic analysis of VP4. Isolated in gnotobiotic pigs. Preparation is of gut origin and its viral ancestry is of gut origin only.

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