ATCC® Number:VR-1558™
Classification: Nidovirales, Coronaviridae, Coronavirus, Group 2
Agent: Human coronavirus OC43
Strain: TC-adapted OC43
Original Source: derived from existing strain (This virus was tissue culture adapted by Kapikian from a mouse preparation of OC43 which was isolated from a man with common cold-like illness.)
Depositors: JO Fleming
Biosafety Level:2
Shipped: frozen
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Tested Positive for Mycoplasma : Yes
Host Organism : HCT-8 ATCCCCL-244
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 33.0°C Duration: 4-5 days with rocking; For best results, cells should be 24-48 hours old and 70-90% confluent (not 100% confluent).
Effect : Yes, in vitro effects: Cytopathic effects (vacuolization of cytoplasm)
Store at : -70°C (or colder)
