ATCC? Number:VR-1310?
Classification: Chlamydiacea, Chlamydophila
Agent: Chlamydophila pneumoniae deposited as Chlamydia pneumoniae Grayston et al.
Strain: TWAR strain CDC/CWL-029 [strain TWAR (CDC/CWL-029)]
Original Source: Throat swab of pneumonia patient
Depositors: RC Barnes
Biosafety Level:2
Shipped: frozen
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Host Organism : HEp-2 cells (ATCCCCL-23) HeLa 229 cells (ATCCCCL-2.1)
Incubation : Duration: 3 days at 35-37.0°C in HEp-2 cells (ATCCCCL-23)
Effect : Yes, in vitro effects: Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Store at : -70.0°C or lower
Comments : The C. pneumoniae strains are at times referred to as TWAR strains after TW-183 (ATCCVR-2282) and AR-39 (ATCC53592). Cytotoxic (nonproductive) at high MOI. Intracellular inclusions contain no glycogen (iodine stain negative). Inclusions will stain with monoclonals against the LPS (genus antigen) and monoclonals directed specifically against C. pneumoniae.
