ATCC® Number:VR-1354™
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: WR (NIH TC-adapted)
Original Source: multiple laboratory passages in cell culture of WR strain of vaccinia
Depositors: NS Cooper, B Moss
Biosafety Level:2
Shipped: frozen
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Incubation : Temperature: 37.0°C Duration: 2-3 days at 37C
Effect : Yes, in vitro effects: Cytopathic effects (localized enlarged, rounded cells which eventually extend throughout the monolayer)
Comments : This strain is recommended by ATCCfor use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified. The host range includes many mammalian and avian cells, e.g.,HeLa S3 (ATCCCCL-2.2) and BS-C-1 (ATCCCCL-26). This tissue culture adapted WR strain of vaccinia is derived from ATCCVR-119 and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984) Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986). Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). Cell associated virus from infected HeLa cells
Related Products: recommended serum:ATCC 30-2020
References : 32840: Salzman NP, Sebring ED. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J. Virol. 1: 16-23, 1967. PubMed: 4248263 32841: Mackett M, et al. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. Sci. USA 79: 7415-7419, 1982. PubMed: 6296831
