Murid herpesvirus 4 (ATCC® VR-1465™)
Permits and Restrictions
View Permits
ClassificationHerpesviridae, Rhadinovirus
Deposited AsMurine gammaherpesvirus 68
AgentMurid herpesvirus 4
StrainWUMS
Biosafety Level2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Formatfrozen 1 mL per vial
Storage Conditions-70℃ or colder
CommentsGenBank: U97553
Effect on Host
CPE, cell rounding and clustering
Recommended Host
BALB/3T12-3 (ATCC® CCL-164™)
Growth Conditions
Temperature: 37℃
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37℃ in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days
Effect on Host
CPE, cell rounding and clustering
Name of DepositorKE Weck, University of Washington School of Medicine
Special CollectionNCRR Contract
SourcePool of lung, spleen, heart, liver, kidney from bank vole (Clethrionomys glareolus), Czechoslovakia, 1976
Geographical IsolationBratislava area
References
Blaskovic D, et al. Isolation of five strains of herpesviruses from two species of free living small rodents. Acta Virol. 24: 468, 1980. PubMed: 6111210
Efstathiou S, et al. Cloning and molecular characterization of the murine herpesvirus 68 genome. J. Gen. Virol. 71: 1355-1364, 1990.
Mackett M, et al. Genetic content and preliminary transcriptional analysis of a representative region of murine gammaherpesvirus 68. J. Gen. Virol. 78: 1425-1433, 1997. PubMed: 9191940
Virgin HW, et al. Complete sequence and genomic analysis of murine gammaherpesvirus 68. J. Virol. 71: 5894-5904, 1997. PubMed: 9223479
Weck KE, et al. Murine gamma-herpesvirus 68 causes severe large-vessel arteritis in mice lacking interferon-gamma responsiveness: a new model for virus-induced vascular disease. Nat. Med. 3: 1346-1353, 1997. PubMed: 9396604
Cross References
Nucleotide (GenBank) : U97553 Murine herpesvirus 68 strain WUMS, complete genome
