ATCC® Number:12982™
Organism: Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
Isolation: Oncopeltus fasciatus (?), 1926
Depositors: HN Guttman
History: ATCC<<--HN Guttman<<--M. Lwoff <<--- C.M. Wenyon <<--- H. Noguchi
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 483: Guttman's IIB mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Composition of paraflagellar rod [5046] monophyly of endosymbiont-containing trypanosomatids [38603] Ultrastructure of symbiotic bacteria [4780] Riboprinting and taxonomy [23607] Effects of methylglyoxal bis (guanylhydrazone) on growth [4792] Cyclopropane fatty acid [24043] Reduced growth of symbiotes free cells [24105] Symbiote-free hemoflagellates, liver factor requirement and serologic identity [24145]
Cross References: Nucleotide (GenBank) : AF060882 Crithidia oncopelti kinetoplast NADH dehydrogenase subunit 8 (ND8) and Cyb gRNA genes, complete cds.
References: 4780: Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371 4792: Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567 4856: . . Exp. Parasitol. 14: 129-142, 1963. 5046: Schlaeppi K, et al. The major component of the paraflagellar rod of Trypanosoma brucei is a helical protein that is encoded by two identical, tandemly linked genes. J. Cell Biol. 109: 1695-1709, 1989. PubMed: 2793936 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23824: Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24105: Chang KP. Reduced growth of Blastocrithidia culicis and Crithidia oncopelti freed of intracellular symbiotes by chloramphenicol. J. Protozool. 22: 271-276, 1975. PubMed: 807721 24145: Chang KP. Symbiote-free hemoflagellates, Blastocrithidia culicis and Crithidia oncopelti: their liver factor requirement and serologic identity. J. Protozool. 23: 241-244, 1976. PubMed: 933081 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198 38603: Hollar J, et al. Monophyly of endosymbiont containing trypanosomatids: Phylogeny versus taxonomy. J. Eukaryot. Microbiol. 45: 293-297, 1998. PubMed: 9627990
ATCC12982
