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平臺編號：bio-48740
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國際編號：ATCC 30023
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拉丁屬名： Trypanosoma equiperdum
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
NIH dyskinetoplastic
ATCC? Number:30023?
Organism: Trypanosoma equiperdum Doflein
Designations: NIH dyskinetoplastic
Isolation: 1903 (?)
Depositors: EJ Tobie
History: ATCC<<--EJ Tobie<<--USDA <<--- Pasteur Institut
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Chromosomes [4840] Kinetoplast DNA in dyskinetoplastic strains [4907]
Classification: KINGDOM: Protozoa
References: 4840: Van der Ploeg LH, et al. Chromosomes of kinetoplastida. EMBO J. 3: 3109-3115, 1984. PubMed: 6526012 4907: Hajduk SL. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science 191: 858-859, 1976. PubMed: 1251198 5001: Tobie EJ. Loss of the kinetoplast in a strain of Trypanosoma equiperdum. Trans. Am. Microsc. Soc. 70: 251-254, 1951.
ATCC30023
Organism: Trypanosoma equiperdum Doflein
Designations: NIH dyskinetoplastic
Isolation: 1903 (?)
Depositors: EJ Tobie
History: ATCC<<--EJ Tobie<<--USDA <<--- Pasteur Institut
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Chromosomes [4840] Kinetoplast DNA in dyskinetoplastic strains [4907]
Classification: KINGDOM: Protozoa
References: 4840: Van der Ploeg LH, et al. Chromosomes of kinetoplastida. EMBO J. 3: 3109-3115, 1984. PubMed: 6526012 4907: Hajduk SL. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science 191: 858-859, 1976. PubMed: 1251198 5001: Tobie EJ. Loss of the kinetoplast in a strain of Trypanosoma equiperdum. Trans. Am. Microsc. Soc. 70: 251-254, 1951.
ATCC30023
