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ATCC? Number:30038?
Organism: Trypanosoma mega Dutton and Todd
Isolation: toad, Bufo regularis, Africa
Depositors: HN Guttman
History: ATCC<<--HN Guttman<<--M. Steinert
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Phylogenetic relationships among anuran trypanosomes  [4677] Polyamine function  [23571] Cysteine and metalloproteinase activities  [23887] Genus identification based on specific 18SRibosomal RNA sequence  [23914] Polyamines  [23962] Karyotype analysis of anuran trypanosomes  [24051] enzymes of ornithine-arginine metabolism  [24117] Glycosphingolipid  [24151] Proteolytic activities  [23776] Acetylornithinase and ornithine acetyltransferase  [24012]
Cross References: Nucleotide (GenBank) : AJ223567 Trypanosoma mega, small subunit ribosomal RNA gene.
Classification: KINGDOM: Protozoa
References:  4677: Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting.  J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144 4890: Nature 178: 308-309, 1956. 23571: Bacchi CJ, et al. Polyamine function in trypanosomatids: activation of cytoplasmic alpha-glycerophosphate dehydrogenase by cationic trypanocides.  Adv. Polyamine Res. 2: 129-138, 1978. 23614: Lopez TD. Volumen celular y adn de Tripanosomas.  Acta Macarao 1(1): 1-12, 1973. 23616: Lopez TD. Volumen celular y contenido de adn de Trypanosoma lewisi y T. mega in vitro.  Acta Macarao 1(1): 13-20, 1973. 23776: Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids.  J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 23887: Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids.  J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943 23914: Uliana SR, et al. Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence.  Exp. Parasitol. 72: 157-163, 1991. PubMed: 2009920 23962: Bacchi CJ, et al. Polyamines in trypanosomatids.  J. Bacteriol. 131: 657-661, 1977. PubMed: 885842 24012: Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase.  Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 24051: Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis.  J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044 24117: Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism.  Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593 24151: Vermelho AB, et al. Isolation and characterization of a neutral glycosphingolipid from the epimastigote form of Trypanosoma mega.  J. Protozool. 33: 208-213, 1986. PubMed: 3735150