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Herpetomonas megaseliae Daggett et al.拉丁名

 (ATCC? 30209?) 統(tǒng)一編號(hào)

Application 用途 Phylogenetic analysis of RNA editing 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 

Type Strain 模式菌株 yes

Comments 注釋 species description； 

Multiplication in tomatoes； 

Distribution of carbohydrate epitopes； 

Monoclonal antibodies for identification； 

Physiological alterations accompanying infectivity； 

Comparative physiology； 

Phylogenetic analysis of RNA editing； 

Proteolytic activities； 

Acetylornithinase and ornithine acetyltransferase； 

Growth on blood-agar plates； 

Effects of hydroxyurea on morphology and growth； 

Cyclopropane fatty acid； 

Ultrastructural observations； Unique no-branched polyunsaturated fatty acids； isoleucine requirement and threonine deaminase； endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification 

Medium 培養(yǎng)基 ATCC? Medium 1011: Diphasic blood agar medium 

ATCC? Medium 1011: Diphasic blood agar medium 

ATCC? Medium 706: M medium 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 25.0°C 

Duration: axenic 

Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. 

Subcultivation Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. 

Name of Depositor J Janovy 

References Yoshida N. Herpetomonas mariadeanei sp. n. (Protozoa, Trypanosomatidae) from Muscina stabulans (Fallen, 1816) (Diptera, Muscidae). J. Protozool. 25: 421-425, 1978. Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. 

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421 

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148 

Daggett PM. Herpetomonas megaseliae sp. n. (Protozoa: Trypanosomatidae) from megaselia scalaris (Loew, 1866) Schmitz, 1929 (Diptera: Phoridae)*. J. Parasitol. 58: 946-949, 1972. 

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. 

Teixeira MM, et al. Characterization of the target antigens of Phytomonas-specific monoclonal antibodies. J. Eukaryot. Microbiol. 42: 232-237, 1995. Daggett PM, Decker JE. Some physiological alterations accompanying infectivity to mammals by four genera of Trypanosomatidae. Comp. Biochem. Physiol. 59: 363-366, 1978. Janovy J Jr.. Problems in the comparative physiology of some trypanosomatid flagellates. Acta Trop. 34: 177-184, 1977. PubMed: 19960 Landweber LF, Gilbert W. Phylogenetic analysis of RNA editing: a primitive genetic phenomenon. Proc. Natl. Acad. Sci. USA 91: 918-921, 1994. PubMed: 8302867 Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 Keppel AD, Janovy J J. Herpetomonas megaseliae and Crithidia harmosa: growth on blood-agar plates. J. Parasitol. 63: 879-882, 1977. PubMed: 562401 

Knight SA. Differentiation of Herpetomonas megaseliae: effects of hydroxyurea on morphology and growth. J. Parasitol. 62: 515-522, 1976. PubMed: 957031 

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 

Janovy J J, et al. The differentiation of Herpetomonas megaseliae: ultrastructural observations. J. Protozool. 21: 53-59, 1974. PubMed: 4817982 

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937 

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116 

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 

Landweber LF, Gilbert W. RNA editing as a source of genetic variation. Nature 363: 179-182, 1993. PubMed: 8387160 type strain 

Cross References Nucleotide (GenBank) : U01006 Herpetomonas megaseliae kinetoplast 9S rRNA gene. 

Nucleotide (GenBank) : U01010 Herpetomonas megaseliae kinetoplast 12S rRNA gene. Nucleotide (GenBank) : U01014 Herpetomonas megaseliae 16S-like small subunit rRNA. 

Nucleotide (GenBank) : L10848 kinetoplast cytochrome oxidase subunit III (COIII) mRNA, complete coding sequence