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NP 4
    NP 4
  • 平臺編號:bio-48475
  • 國際編號:ATCC 30346
  • 拉丁屬名: Crypthecodinium cohnii
  • 規(guī)格:frozen
  • 用途:ATCC原裝進口
  • 服務費用:
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  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產品信息以出庫為準)

ATCC? Number:30346?
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: NP 4
Isolation: miscellaneous plankton, Long Island Sound, NY, 1974
Depositors: CA Beam, M Himes
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 460: A2E6 mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Major Sibling Species WH genetic diversification [4790] [4783] cryopreservation [23828] use of plastic ampoules for freeze preservation [23870]
Classification: KINGDOM: Protozoa
References: 4783: Salt GW. Changes in the cell volume of Didinium nasutum during population increase. J. Protozool. 22: 112-115, 1975. 4790: Beam CA, Himes M. Sexual isolation and genetic diversification among some strains of Crypthecodinium cohnii-like dinoflagellates evidence of speciation. J. Protozool. 24: 532-539, 1977. 23828: Simione FP Jr., Daggett PM. Recovery of a marine dinoflagellate following controlled and uncontrolled freezing. Cryobiology 14: 362-366, 1977. PubMed: 560941 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

ATCC30346

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