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Crypthecodinium cohnii (Seligo) Javornicky 拉丁名

 (ATCC? 30773?) 統(tǒng)一編號

Strain Designations 別名 MBS2

Application 用途 Biofuel production

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation 分離基物 Sargassum sp., Miami Beach, FL, 1977

Product Format 提供形式 frozen

Type Strain 模式菌種 no

Comments 注釋 Major Sibling Species Y

geographical distribution

Medium培養(yǎng)基 ATCC? Medium 460: A2E6 medium

Growth Conditions 生長條件

Max Temperature 最高培養(yǎng)溫度: 25.0°C

Min Temperature 最低培養(yǎng)溫度: 20.0°C

Duration: axenic

Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.

Subcultivation

Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.

Name of Depositor 寄存者 CA Beam, M Himes

Year of Origin 收藏年份 1977

References 參考文獻 Beam CA, Himes M. Distribution of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 29: 8-15, 1982.