ATCC® Number:30871™
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: CCAP 1501/3a
Isolation: freshwater, Drummond, WI, 1964
Depositors: CCAP
History: ATCC<<--CCAP<<--F.C. Page 23
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Growth condition: Axenic Protocol: This strain is distributed as a frozen preparation. See the general procedures for thawing a frozen vial. Once vial is thawed, aseptically transfer the entire contents to a 16 X 125 mm screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of ATCCmedium 712. Incubate horizontally at 25C. Trophozoites should be evident in 1-5 days.
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Comments: Re-definition of the genus [4757] mitochondrial DNA fingerprinting [21722] Restriction fragment length polymorphisms of DNA [5037] phylogeny [24028] characterization of Acanthamoeba polyphaga [24209]
Cross References: Nucleotide (GenBank) : AF019061 Acanthamoeba polyphaga Page-23 18S ribosomal RNA gene, partial sequence.
Classification: KINGDOM: Protozoa
References: 4757: Page FC. Re-definition of the genus Acanthamoeba with descriptions of three species. J. Protozool. 14: 709-724, 1967. PubMed: 5604481 5037: McLaughlin GL, et al. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amebae. J. Clin. Microbiol. 26: 1655-1658, 1988. PubMed: 2903176 21722: Gautom RK, et al. Mitochondrial DNA fingerprinting of Acanthamoeba spp. isolated from clinical and environmental sources. J. Clin. Microbiol. 32: 1070-1073, 1994. PubMed: 7913095 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 24209: Fritsche TR, et al. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J. Clin. Microbiol. 31: 1122-1126, 1993. PubMed: 8501212 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 33455: Kong HH, et al. Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga. Korean J. Parasitol. 33: 331-340, 1995. PubMed: 8591011 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 70201: Alves JMP, et al. Random amplified polymorphic DNA probes as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz. J. Med. Biol. Res. 33: 19-26, 2000. 70296: Khan NA. Pathogenicity, morphology, and differentiation of Acanthamoeba. Curr. Microbiol. 43: 391-395, 2001. PubMed: 11685503 70297: Khan NA, Paget TA. Molecular tools for speciation and epidemiological studies of Acanthamoeba. Curr. Microbiol. 44: 444-449, 2002. PubMed: 12000996 89073: Khan NA, Tareen NK. Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains. Folia Parasitol. 50: 97-104, 2003. PubMed: 14560969
ATCC30871
