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平臺編號：bio-47515
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國際編號：ATCC 50506
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拉丁屬名： Encephalitozoon intestinalis
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Encephalitozoon intestinalis
ATCC? Number:50506?
Organism: Encephalitozoon intestinalis (Cali et al.) Hartskeerl et al.
Designations: alveolar isolate
Isolation: alveolar material from HIV-seropositive human, New York City, NY, 1994
Depositors: ES Didier
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mycoplasma free.
References: 18381: Didier ES, et al. Identification and characterization of three Encephalitozoon cuniculi strains. Parasitology 111: 411-421, 1995. PubMed: 11023405 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs. J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 18384: Didier ES, et al. Diagnosis of disseminated microsporidian Encephalitozoon hellum infection by PCR - southern analysis and successful treatment with albendazole and fumagillin. J. Clin. Microbiol. 34: 947-952, 1996. PubMed: 8815114 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 24196: Doultree JC, et al. In vitro growth of the microsporidian Septata intestinalis from an AIDS patient with disseminated illness. J. Clin. Microbiol. 33: 463-470, 1995. PubMed: 7714208
ATCC50506
Organism: Encephalitozoon intestinalis (Cali et al.) Hartskeerl et al.
Designations: alveolar isolate
Isolation: alveolar material from HIV-seropositive human, New York City, NY, 1994
Depositors: ES Didier
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mycoplasma free.
References: 18381: Didier ES, et al. Identification and characterization of three Encephalitozoon cuniculi strains. Parasitology 111: 411-421, 1995. PubMed: 11023405 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs. J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 18384: Didier ES, et al. Diagnosis of disseminated microsporidian Encephalitozoon hellum infection by PCR - southern analysis and successful treatment with albendazole and fumagillin. J. Clin. Microbiol. 34: 947-952, 1996. PubMed: 8815114 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 24196: Doultree JC, et al. In vitro growth of the microsporidian Septata intestinalis from an AIDS patient with disseminated illness. J. Clin. Microbiol. 33: 463-470, 1995. PubMed: 7714208
ATCC50506
