ATCC® Number:50906™
Organism: Perkinsus marinus (Mackin et al.) Levine
Designations: LA10-1 [LA-10-1]
Isolation: clone derived from strain LA10 (ATCC50772), which came from eastern oyster, Crassotrea virginica, Louisiana Gulf Coast, 1998
Depositors: D Bushek
History: ATCC<<--D Bushek<<--K. Hudson
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1886: Perkinsus broth mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 50439 SPEC: When the frozen ampule arrives, place it directly into a 35C-water bath and transfer its thawed contents to 14 ml of fresh medium in a T-75 tissue culture flask. Maintain by removing 13 ml of cell suspension weekly and replacing with an equal volume of fresh medium. Alternately, aseptically transfer 1.0 ml of a growing culture to 13 ml of fresh medium in a T-75 tissue culture flask. Please note: This strain has wide tolerances to most environmental variables, i.e., temperature range: 15-35C, salinity range: 10-60 parts per thousand; pH range: 6.0-8.5. The distribution of the species worldwide is not known. In order to avert the introduction of this pathogen into non-endemic areas, all culture wastes must be treated as biohazardous and autoclaved prior to disposal. There are no known mechanisms for eradication of this pathogen from the environment.
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Comments: parasite of eastern oyster, Crassotrea virginica, and possibly several other marine bivalves This strain is useful for studies of population genetics, systematics, and virulence.
Classification: KINGDOM: Protozoa
ATCC50906
