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平臺編號：bio-47041
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國際編號：ATCC PRA-113
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拉丁屬名： Acanthamoeba hatchetti
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Acanthamoeba hatchetti
ATCC? Number:PRA-113?
Organism: Acanthamoeba hatchetti Sawyer et al.
Designations: 2HH
Isolation: clinical specimen - human (cornea of patient with keratitis)Vienna Austria Isolation date: 1996
Depositors: J Walochnik
History: J Walochnik
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain. Interval: every month
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
References: 90184: Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 90185: Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 90212: Hiti K, et al. Microwave treatment of contact lens cases contaminated with acanthamoeba. Cornea 20: 467-470, 2001. PubMed: 11413399
Organism: Acanthamoeba hatchetti Sawyer et al.
Designations: 2HH
Isolation: clinical specimen - human (cornea of patient with keratitis)Vienna Austria Isolation date: 1996
Depositors: J Walochnik
History: J Walochnik
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain. Interval: every month
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
References: 90184: Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 90185: Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 90212: Hiti K, et al. Microwave treatment of contact lens cases contaminated with acanthamoeba. Cornea 20: 467-470, 2001. PubMed: 11413399
