Human metapneumovirus
VR-3282 ™
Human metapneumovirus strain A/WI/629-D02421/2010 is propagated in LLC-MK2 derivative cells (ATCC CCL-7.1). This culture was isolated in 2010 from a human respiratory swab in Milwaukee, Wisconsin.
Product category
Viruses
Product type
Animal virus
Strain designation
A/WI/629-D02421/2010
Common name
hMPV
Isolation source
Human respiratory swab
Geographical isolation
United States; Wisconsin; Milwaukee
Applications
Infectious disease research
Respiratory disease research
Assay development
Product format
Frozen
Storage conditions
-70°C or colder
Handling information
Host
LLC-MK2 Derivative (ATCC CCL-7.1)
Effects
cell rounding; cell sloughing; cell detachment
Complete medium
EMEM (ATCC 30-2003) + 10 BAEE/mL TPCK-trypsin (Sigma T1426 or equivalent)
Temperature
37°C
Incubation
7-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 30-60% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 90-100% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
Deposited as
Human metapneumovirus
Depositors
K Henrickson, Children’s Hospital of Wisconsin
Type of isolate
Human
Year of origin
2010
Legal disclaimers
