Product category
Fungi
Product type
Yeast
Strain designation
CBS 7877 [ATCC 96810, JCM 14890, RA 42.2C, NBRC 103918]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Skin
Geographical isolation
England; Bristol
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Characteristics
Comments
Lipophilic or lipid-dependent yeasts.
Genome sequencing strain (The Procter & Gamble Company, USA; Genome Institute of Singapore, Singapore).
Opportunistic pathogen that can cause systematic infection in neonates and are associated with multiple human skin disorders.
Handling information
Medium
ATCC Medium 2693: modified Dixon (mDixon)
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25?°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 50 µL (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Lipophilic or lipid-dependent yeasts. Genome sequencing strain (The Procter & Gamble Company, USA). Opportunistic pathogen that can cause systematic infection in neonates and are associated with multipGeneral
Preceptrol
No
Characteristics
Comments
Lipophilic or lipid-dependent yeasts.
Genome sequencing strain (The Procter & Gamble Company, USA; Genome Institute of Singapore, Singapore).
Opportunistic pathogen that can cause systematic infection in neonates and are associated with multiple human skin disorders.
Handling information
Medium
ATCC Medium 2693: modified Dixon (mDixon)
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25?°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 50 µL (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Lipophilic or lipid-dependent yeasts. Genome sequencing strain (The Procter & Gamble Company, USA). Opportunistic pathogen that can cause systematic infection in neonates and are associated with multiple human skin disorders.
If having trouble reviving this strain, try using liquid media with shaking; subculture at intervals of 2 weeks after recovering.
Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.le human skin disorders.
If having trouble reviving this strain, try using liquid media with shaking; subculture at intervals of 2 weeks after recovering.
Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.
