Product category
Bacteria
Strain designation
87-66
Type strain
No
Genome sequenced strain
Yes
Isolation source
Blood of man with AIDS
Geographical isolation
United States; Oklahoma; Oklahoma City
Applications
Infectious disease research
Vector-borne disease research
Product format
Freeze-dried
Storage conditions
2°C to 8°C
Specific applications
Characterization
Preceptrol
No
Characteristics
Comments
Case report, patient 1
Handling information
Medium
ATCC Medium 18: Trypticase Soy Agar/Broth
ATCC Medium 1814: Rabbit heart infusion agar
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Open vial.
Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #1814 agar slant and/or plate.
Incubate the tubes and plate at 37°C under 5% CO2 for 7 to 10 days. Good growth should be obtained in the broth pool at the bottom of the slant. Colony formation may take longer. Further subcultures can be made using the broth pool as the inoculum source.
Handling notes
This is a slow-growing organism that requires moist conditions for best growth. Growth at the broth/agar interface of the biphasic slant should occur within 7-10 days. The use of FRESH media is of primary importance.
Medium #4 (Tryptic Soy Agar with 5% Defibrinated Rabbit Blood) may also be used. Once good growth is obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or “ultra-low temperature” freezer, is recommended.
Verification method
Whole-genome Sequencing
History
Deposited as
Rochalimaea henselae Regnery et al.
Depositors
DF Welch
Chain of custody
ATCC <-- DF Welch <-- D Hensel
Type of isolate
Human
Patient age
31 years
Patient gender
Male
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
