Campylobacter fetus subsp. fetus (Smith and Taylor) Veron and Chatelain
BAA-2152 ™
Product category
Bacteria
Strain designation
82-40
Type strain
No
Genome sequenced strain
Yes
Geographical isolation
United States; Pennsylvania; Pittsburgh
Applications
Agricultural research
Product format
Freeze-dried
General
Preceptrol
No
Characteristics
Comments
Genome sequenced strain
Handling information
Medium
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate additional #260 broth tubes, an agar slant, and/or plate.
5. Incubate the tubes and plate at 37°C under microaerophilic conditions for 72 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
Handling notes
After 72 hours, colonies on blood plates are small, smooth, gray, transparent, and nonhemolytic.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
Depositors
M Blaser
Type of isolate
Human
Year of origin
1981
Cross references
GenBank CP000487 Campylobacter fetus subsp. fetus 82-40, complete genome
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