Product category
Viruses
Product type
Animal virus
Classification
Coronaviridae, Coronavirus, Group 1
Strain designation
CCV-TN449
Common name
CCV
Genome sequenced strain
Yes
Isolation source
Dog which died of gastroenteritis; propagated and attenuated in CRFK cell cultures
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Respiratory research
Characteristics
Mycoplasma contamination
Not detected
Handling information
Host
CRFK (ATCC CCL-94)
Effects
CPE; syncytia
Complete medium
EMEM (ATCC 30-2003) + 1.0 μg/mL Gibco Trypsin (1:250) Powder (Thermo Fisher Scientific Cat. # 27250-018)
Temperature
35-38°C
Atmosphere
95% Air, 5% CO2
Incubation
1 to 4 days at 37°C, rocking at setting #1, a 5% CO2 in air atmosphere is recommended.
Recommendation for infection
For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Handling notes
TN-449 is an attenuated strain of canine coronavirus cited in U.S. Patents 5,047,238 and 5,012,663 as efficacious when administered parenterally to dogs as a live or inactivated vaccine.
Key abbreviations
CO2, Carbon dioxide
CPE, Cytopathic effect
CRFK, Crandell feline kidney cells
EMEM, Eagle's Minimum Essential Medium
FA, Fluorescent antibody assay
µg, Microgram
mL, Milliliter
TC, Tissue culture
TCID[50], Median tissue culture infective dose
U.S., United States of America
VMRD, Veterinary Medical Research and Development
