Penicillium ludwigii Udagawa
9112 ™
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Drug-resistant fungus
Classification
Fungi, Dikarya, Ascomycota, Pezizomycotina, Eurotiomycetes, Eurotiomycetidae, Eurotiales, Aspergillaceae, Penicillium
Strain designation
USDA 1336.2 [ATCC 9824, CBS 110.66, IMI 61271, NRRL 744, QM 477, VKM F-1827]
Type strain
No
Applications
Antimicrobial resistance research
Drug development
Product format
Freeze-dried
Storage conditions
2°C to 8°C
Specific applications
Fungus resistance testing
Fungus resistance testing aircraft transmissions
Fungus resistance testing plastics
Fungus resistance testing textiles
Produces glucan endo-1,6-beta-glucosidase beta-D-1,6 glucanase
Produces purine beta-ribonucleosidases
Resistant to copper
Testing
Preceptrol
No
Characteristics
Comments
The strain was re-identified as Penicillium ludwigii, a close sister of P. ochro-chloron and P. janthinellum, based on the multigene sequence analysis. This change does not affect to its use as quality control strain for standard tests.
Handling information
Medium
ATCC Medium 336: Potato dextrose agar (PDA)
ATCC Medium 312: Czapek's agar
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2 to 4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
