Clostridioides difficile (Prevot) Lawson et al.
BAA-1805 ™
Download GenomeLearn about the ATCC Genome Portal This strain of Clostridioides difficile is a clinical isolate with applications in enteric disease research. This strain is toxinotype IIIB and is positive for the presence of the cdtB, tcdA, and tcdB genes.
Product categoryBacteria
Product typeAnaerobe
Type strainNoGenome sequenced strainYesIsolation sourceClinical isolateApplicationsEnteric disease research
Infectious disease research
Product formatFreeze-driedStorage conditions2°C to 8°C
GenotypeToxinotype: IIIbToxigenicYesToxin genescdtB (Binary toxin) positive
tcdA (Toxin A) positive
tcdB (Toxin B) positive
CommentsPresence of tcdA and tcdB genes confirmed by PCR. Ref
Confirmed by CDC: NAP1, Toxinotype III, binary toxin positive
Binary Toxin gene cdtB was amplified by PCR Ref
Toxinotype IIIb
Ribotype 027
MediumATCC Medium 2107: Modified Reinforced Clostridial
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature37°C
AtmosphereAnaerobic
Handling procedureOpen vial.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24-48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
