Campylobacter jejuni subsp. jejuni (Jones et al.) Steele and Owen
35924 ™
Product category Bacteria
Strain designation BG 263 [C2179]
Type strain No
Applications
Enteric disease research
Infectious disease research
Product format Freeze-dried
Specific applications
Bacteriophage host
Enteric Research
Emerging infectious disease research
Preceptrol No
Comments
Phage typing system
Medium
ATCC Medium 1423: Modified Brucella broth
ATCC Medium 1423: Modified Brucella broth
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Microaerophilic
Handling procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #1115 or #177 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 slant, and/or plate.
5. Or, to obtain a biphasic culture, add 0.5 ml of the suspension to a #260 agar slant (see notes).
6. Incubate tubes and plate at 37oC, under microaerophilic conditions, for 24 to 48 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.