Spathaspora passalidarum Nguyen et al.
MYA-4345 ™
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Strain designation
11-Y1 [CBS 10155, NRRL Y-27907]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Gut of Odontotaenius disjunctus (Passalidae)
Geographical isolation
United States; Louisiana; West Feliciana Parish
Applications
Biofuel production
Product format
Frozen
Storage conditions
-80°C or colder
General
Specific applications
Ferments pentose (xylose).
Bioethanol production
Potential biofuel production agent
Preceptrol
No
Characteristics
Morphology
After 6 days on YM medium at 25°C, colonies are white, smooth, butyrous. Cells are globose or subglobose, single or with bud. Pseudohyphae are not observed.
Comments
Ferments pentose (xylose)
Insect associated yeast
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA)
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Quality control specifications
History
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