Escherichia coli (Migula) Castellani and Chalmers
23506 ™
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Product category
Bacteria
Classification
Enterobacteriaceae, Escherichia
Strain designation
NCDC Bi 8337-41
Type strain
No
Genome sequenced strain
Yes
Isolation source
Peritonitis
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Preceptrol
No
Characteristics
Serotype
O10:K5(L):H4
Handling information
Medium
ATCC Medium 3: Nutrient agar or nutrient broth
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
Open vial according to enclosed instructions.
Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
Colonies on # 3 agar are entire, glistening, circular, smooth, low convex, and translucent. Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
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