Specific applicationstransformation host
PreceptrolNo
CommentsPlasmid host
Host for pVA838 (ATCC 37160).
Genome sequenced strain
Medium 培養(yǎng)基 ATCC Medium 44: Brain Heart Infusion Agar/Broth
ATCC
Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature 培養(yǎng)溫度 37°C
Atmosphere 需氧情況 95% Air, 5% CO2
Handling procedureOpen thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Aseptically transfer the entire contents to a 5-6 mL tube of #44 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate at 37°C for 24 hours in an atmosphere of 5% CO2.
Verification methodWhole-genome Sequencing
Deposited asStreptococcus sanguis White in White and NivenDepositorsFL MacrinaCross referencesGenBank CP000725 Streptococcus gordonii str. Challis substr. CH1, complete genome.