MITC-STO (ATCC 56-X)
56-X.2 ™
MITC-STO mitomycin C-treated feeder cells are fibroblasts derived from a mouse embryo. These cells can be used as feeder cells to support the growth of other cells. This cell line has applications in stem cell research.
Product category
Animal cells
Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Cell type
fibroblast
Morphology
fibroblast
Tissue
Embryo
Applications
Stem cell research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
General
Specific applications
Mitomycin C-treated cells are used as a feeder layer
Characteristics
Growth properties
Adherent
Age
embryo
Comments
These cells are generated from mouse embryonic fibroblasts, STO cell line (ATCC CRL-1503), by a treatment of Mitomycin C.
These cells are provided to be used as feeder cells to support the growth of other cells. They have been treated with Mitomycin C and will not replicate. The cells will begin to deteriorate in 2 to 3 weeks after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. Such populations are employed for maintenance of embryonal stem cells such as ES-D3 (ATCC CRL-1934) or teratocarcinoma stem cells (see ATCC CRL-1535 and ATCC CRL-1566) in the undifferentiated state.
It is recommended that the feeder cells be plated 24 hours before use at 6 x 106/T-75 or 2 x 106/T-25 in order to obtain a 100% confluent monolayer for stem cells growth.
Feeder layers are also employed to enhance the growth at low density populations of many hybridomas, colon carcinoma cell line (ATCC CCL-247) and squamous cell carcinoma cell lines (ATCC CRL-1624 and ATCC CRL-1629). This action is due partly to conditioning of the substrate and the medium. A feeder layer confluence of 30 % is adequate and can be obtained by plating 2 x 106/T-75.
Handling information
Quality control specifications
History
Legal disclaimers
