Malassezia furfur (Robin) Baillon
MYA-4874 ™
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Strain designation
M47
Type strain
No
Isolation source
Face of patient with atopic dermatitis
Geographical isolation
Lithuania; Vilnius
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Characteristics
Morphology
On modified Leeming & Notman medium at 34°C after 14 days, colonies buff to tan, raised, smooth to wrinkled, margin lobed. Cells elongated ellipsoidal, smooth, 1.5-6 x 1.5-2.25 µm. Pseudohyphae not observed.
Comments
Clinical isolate
Lipophilic, but able to produce small colonies on Sabouraud agar without supplementation of lipids
Handling information
Medium
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
ATCC Medium 2693: modified Dixon (mDixon)
Temperature
30-34°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 50 µL (or 2 to 3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 3 to 4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Clinical isolate; Lipophilic, but able to produce small colonies on Sabouraud agar without supplementation of lipids.
Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.
Quality control specifications
Sequenced data
D1D2 region of the 28S ribosomal RNA gene; GenBank number JQ958299:
GGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGAGGGAAGAGCTCAAATTTGAAAGCTGGTACCTCCGGTATCCGCGTTGTAACCTCGAGACACGTTTTCCGTGCGGCGCTATGGACAAGTTCCTTGGAACAGGACATCGTAGAGGGTGAAAATCCCGTACTTGCCATGGATGTACCGTGCTTTGCGATACGTGCTCTAAGAGTCGAGTTGTTTGGGATTGCAGCTCAAAATGGGTGGTAGACTCCATCTAAAGCTAAATATCGGGGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAGTACGTGAAATTGTCGAAAGGGAAGCGCTTGAAGTCAGCCATGCCGCTCAGGACTCAGCCTGGCTTTTGCTTGGTGTATTTCCTGGGTAGCAAGTCAGCATTGGTTTGGATCGTCGGAGAAGCATAGTCGGAATGTGGCGCCCTCGGGCGTGTTATAGCCTTCTATTGGATACGACGATCTAGACCAAGGAACGCAGCGCGCCTTATGGCGGGTCTTCGGACACCTTCGCGCTTAGGATGCTGGCGTAATGGCTTTAAGTGG
History
Synonyms
Pityrosporum orbiculare Gordon
Depositors
A Zinkeviciene
Chain of custody
ATCC <-- A Zinkeviciene
Type of isolate
Human
Cross references
GenBank JQ958299 D1/D2 region of 28S rRNA gene
Legal disclaimers
