Clostridium perfringens (Veillon and Zuber) Hauduroy et al.
51880 ™
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Product category
Bacteria
Product type
Anaerobe
Strain designation
138/88
Type strain
No
Genome sequenced strain
Yes
Isolation source
Pig intestines with necrotic enteritis
Geographical isolation
Slovenia
Applications
Agricultural research
Vaccine development
Product format
Freeze-dried
General
Specific applications
Used to produce vaccine against beta-toxin
Preceptrol
No
Characteristics
Antigenic properties
Type C
Handling information
Medium
ATCC Medium 1053: Reinforced Clostridial medium (Oxoid CM149)
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of #1053/2107 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents. Aseptically transfer this aliquot back into the broth tube. Mix well.
3. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053/2107 may also be inoculated with 0.2 ml. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of #1053 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053 may also be inoculated with 0.2 ml. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system
hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic
gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that
an anaerobic gas headspace is retained.
Handling notes
In 24 to 48 hours, growth is evident by turbidity and gas formation in the broth and by colonies on the anaerobic agar surfaces. No growth should occur on agar plate incubated aerobically. On #260 plates, colonies are circular, low convex with a rhizoid edge and double-zone beta hemolysis.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Colonies on #260 agar are irregular, flat, undulate and large. No growth should occur on agar plates incubated aerobically.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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