Shewanella algae Simidu et al. emend. Nozue
51181 ™
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Product category
Bacteria
Strain designation
BrY
Type strain
No
Genome sequenced strain
Yes
Isolation source
Sediment from Great Bay estuary
Geographical isolation
United States; New Hampshire
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Reduces iron (ferric, FeIII) ferric iron
Resistant to chromium
Biotechnology
Preceptrol
No
Handling information
Medium
ATCC Medium 2: Marine agar 2216 or marine broth 2216
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Rehydrate the entire pellet with approximately 0.5 mL of #2 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #2 plate and/or #2 agar slant.
Incubate at 30°C for 24 hours.
Handling notes
To demonstrate anaerobic growth by dissimilatory iron (III) reduction, use ATCC Medium #1931 broth.
1931 Broth: Ferric citrate Medium
Ferric citrate, 13.7 g
NaHCO3, 2.5 g
KCl, 0.1 g
NH4Cl, 1.5 g
NaH2PO4, 0.6 g
Wolfe's Vitamin Solution (see below), 10.0 mL
Wolfe's Mineral Solution (see below), 10.0 mL
Sodium lactate, 30.0 mM
Distilled water, 1.0 L
Boil ferric citrate in distilled water to dissolve; cool to room temperature. Adjust to pH 6.6 with 10N NaOH. Add remaining components. Equilibrate medium under 80% N2, 20% CO2. Dispense medium anaerobically under same gas phase. Autoclave anaerobically at 121oC for 15 minutes. Final pH 7.0.
Wolfe's Vitamin Solution:
Biotin, 2.0 mg
Folic acid, 2.0 mg
Pyridoxine HCl, 10.0 mg
Thiamine HCl, 5.0 mg
Riboflavin, 5.0 mg
Nicotinic acid, 5.0 mg
Calcium D-(+)-pantothenate, 5.0 mg
Vitamin B12, 0.1 mg
p-Aminobenzoic acid, 5.0 mg
Thioctic acid, 5.0 mg
Distilled water, 1.0 L
Wolfe's Mineral Solution:
Nitrilotriacetic acid, 1.5 g
MgSO4.7H2O, 3.0 g
MnSO4.H2O, 0.5 g
NaCl, 1.0 g
FeSO4.7H2O, 0.1 g
CoCl2.6H2O, 0.1 g
CaCl2, 0.1 g
ZnSO4.7H2O, 0.1 g
CuSO4.5H2O, 0.01 g
AlK(SO4)2.12H2O, 0.01 g
H3BO3, 0.01 g
Na2MoO4.2H2O, 0.01 g
Distilled water, 1.0 L
Add nitrilotriacetic acid to approximately 500 mL of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.
Whole-genome Sequencing
History
Deposited as
Shewanella algae Simidu et al. emend. Nozue
Depositors
F Caccavo
Type of isolate
Environmental
Cross references
GenBank AF005691 Shewanella alga DNA gyrase beta-subunit (gyrB) gene, partial cds.
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
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