Sporothrix schenckii Hektoen et Perkins
58251 ™
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation
de Perez 2211183
Type strain
No
Genome sequenced strain
Yes
Isolation source
Clinical isolate
Geographical isolation
United States; Puerto Rico
Applications
Agricultural research
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Biomedical Research and Development Material
Preceptrol
No
Characteristics
Comments
Yeast-mycelium transition
Genome sequencing strain (Broad Institute, USA)
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
History
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