Rotavirus A
VR-3392 ™
This product is an ATCC manufactured and accessioned progeny of VR-2551 cited in US Pat. No. 5,626,851.
Product category
Viruses
Product type
Animal virus
Classification
Reoviridae, Rotavirus, Rotavirus A
Strain designation
WI61
Genome sequenced strain
Yes
Isolation source
Human infant
Geographical isolation
United States; Pennsylvania; Philadelphia
Applications
Infectious disease research
Enteric disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Enteric research
Characteristics
Comments
May cause gastroenteritis in infants
Propagate in cells fed with serum-free medium containing 0.5 µg/mL purified trypsin
Handling information
Host
MA-104 Clone 1 (ATCC CRL-2378.1)
Effects
cell rounding; cell sloughing; CPE; plaque formation
Complete medium
DMEM (ATCC 30-2002) + 8 µg/mL Gibco Trypsin (Invitrogen 27250-018)
Temperature
36°C
Atmosphere
95% Air, 5% CO2
Incubation
1 to 4 days at 36°C, a 5% CO2 in air atmosphere is recommended
Recommendation for infection
For best results, cells should be 24 to 48 hours old and 100% confluent.
Handling notes
Cultivate in cell lines (MA-104, CV-1) fed with serum-free medium supplemented with 8 µg/mL trypsin.
Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.
Key abbreviations
bp, Base pairs
CO2, Carbon dioxide
CPE, Cytopathic effect
CV-1, African green monkey kidney cells
DMEM, Dulbecco's Modified Eagle's Medium
ELISA, Enzyme-linked immunosorbent assay
NCBI, National Center for Biotechnology Information
MA-104, Embryonic rhesus monkey kidney cells
µg, Microgram
mL, Milliliter
PCR, Polymerase chain reaction
RT-PCR, reverse-transcriptase polymerase chain reaction
TC, Tissue culture
TCID[50], Median tissue culture infective dose
Quality control specifications
Verification method
Whole-genome Sequencing
History
Deposited as
ATCC accessioned progeny of Rotavirus A strain WI61 cited in US Pat. No. 5,626,851 as VR-2551.
Type of isolate
Human
Patient age
infant
Special collection
NCRR Contract
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
