Megasphaera cerevisiae Engelmann and Weiss
43236 ™
Product category
Bacteria
Product type
Extremophile
Strain designation
DSM 20461 [Sp III 2e]
Type strain
No
Isolation source
Spoiled beer
Geographical isolation
Germany
Product format
Frozen
Storage conditions
-80°C or colder
General
Specific applications
Characterization
Preceptrol
No
Handling information
Medium
ATCC Medium 1528: PY-fructose (ATCC medium 1524) with 10 g fructose per liter of medium
Temperature
26°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.
Under anaerobic conditions, withdraw 0.5 mL of the recommended broth from a single test tube (5 to 6 mL) and rehydrate the vial contents.
Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 mL each from the suspension. Streak several blood plates to check for colonial morphology and purity.
Incubate tubes under an anaerobic atmosphere at 26°C. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
In 24 to 48 hours, growth should be evident by turbidity that settles to the bottom of the broth. No growth should occur on agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
Use of an anaerobic gas chamber, or
Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in anaerobic chamber,
Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Colonies on anaerobic blood plates are pinpoint, punctiform, colorless, and transparent.
Allow the culture to grow in the primary broth tube before transferring it to other tubes.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
History
Deposited as
Megasphaera cerevisiae Engelmann and Weiss
Depositors
DSM
Chain of custody
ATCC <-- DSM <-- H Seidel Sp III 2e
Type of isolate
Food & Beverage
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